All ' 0 1 2 3 4 5 6 7 8 9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ‘ nucleobase (NOO-klee-oh-bays) noun. basic building block of DNA and RNA, composed of cytosine (DNA and RNA), guanine (DNA and RNA), adenine (DNA and RNA), thymine (DNA) and uracil (RNA). Often simply called "base."